FLAT tagged CRISPR/Cas9 nuclease Adenovirus

Name 
Ad-mCherry-FLAG-hCas9
Cat No 
1906
Availability
Immediate
Categories
Adenovirus, CRISPR/Cas9, Over-Expression

Cas9 is the nuclease guided by the crRNA and tracrRNA (or trans-activating crRNA) to cleave specific DNA sequences. A guide RNA (gRNA) can be designed to include a hairpin that mimics the tracrRNA-crRNA complex. Binding specificity is based on the gRNA and a three nucleotide NGG sequence called the protospacer adjacent motif (PAM) sequence. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms.

For site-specific genome editing, the CRISPR/Cas9 system requires the Cas 9 nuclease and the gRNA. This adenovirus expresses a codon-optimized Cas9 nuclease, along with a GFP reporter. It can be used along with guided RNA for genome-editing.

This adenovirus expresses a FLAG tagged Cas9 with a mCherry reporter.

1906
Ad-mCherry-FLAG-hCas9

Ready-to-use FLAT tagged CRISPR/Cas9 nuclease Adenovirus. CRISPR Cas9 adenovirus Ad-Cas9 Ad-mCherry-Cas9 1906

$690.00
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Product Details

Promoter
CAG (ubiquitous)
Reporter
mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Volume (µL)
200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.