human CASP8 shRNA Adenovirus
This is an pre-made gene silencing adenovirus that expresses a shRNA to knockdown human CASP8 gene. The shRNA expression is driven by an U6 promoter.
The knockdown of this human gene was validated by western blot in A549 cells.
1818
Ad-h-CASP8-shRNA
Ready-to-use human CASP8 shRNA Adenovirus. human CASP8 shRNA adenovirus gene knockdown1818
Gene Reference Data
Alternate Names
ALPS2B; CAP4; Casp-8; FLICE; MACH; MCH5
Description (Vector)
CASP8 encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
Gene ID
841
Gene Name (Vector)
caspase 8
Gene Symbol
CASP8
HGNC ID
HGNC:1509
ORF Size (aa)
837
ORF Size (bp)
837 bp
RefSeq ID
NM_033356
RefSeq Synonyms
NM_033358, NM_033356, NM_033355, NM_001228, NM_001080125, NM_001080124, BC068050,
Species
human
UniGene ID
Hs.599762
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.