human CASP3 shRNA Adenovirus

Name 
Ad-h-CASP3-shRNA
Cat No 
1814
Availability
Immediate

This is an pre-made gene silencing adenovirus that expresses a shRNA to knockdown human CASP3 gene. The shRNA expression is driven by an U6 promoter.

The knockdown of this human gene was validated by western blot in A549 cells.

1814
Ad-h-CASP3-shRNA

Ready-to-use human CASP3 shRNA Adenovirus. human CASP3 shRNA adenovirus gene knockdown1814

$995.00
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Product Details

Promoter
U6 ()
Reporter
eGFP
shRNA Knockdown (%)
70
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Volume (µL)
200

Gene Reference Data

Alternate Names
apopain; CASP-3; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; caspase-3; CPP32; CPP-32; CPP32B; cysteine protease CPP32; PARP cleavage protease; procaspase3; protein Yama; SCA-1; SREBP cleavage activity 1
Description (eCommerce)
Caspase 3 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Caspase 3 cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. Caspase 3 is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease [taken from NCBI Entrez Gene (Gene ID: 836)].
Description (Vector)
CASP3 encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
Gene ID
836
Gene Name (eCommerce)
caspase 3
Gene Name (Vector)
caspase 3
Gene Symbol
CASP3
HGNC ID
HGNC:1504
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
834
ORF Size (bp)
834 bp
Protein Name (eCommerce)
Caspase-3
RefSeq ID
NM_004346
RefSeq Synonyms
NM_032991, NM_004346, BC016926,
Research Areas
Alzheimer's Disease,Amyotrophic Lateral Sclerosis (ALS),Apoptosis,BDNF Signaling,Breast Cancer,Cancer,Cardiac Development,Cardiology,Cardiovascular,CD95/FAS Signaling,Cell Cycle,Colorectal Cancer,Cytoskeleton,DNA Damage/Repair,Hippo Signaling,Host-Virus Interactions,Huntington's Disease,Immunology,Lung Cancer,MAPK Signaling,MicroRNAs in Cancer,Mitochondrion,Neurobiology,Neurodegeneration,Neurodevelopment,NFAT Signaling,NGF Signaling,p53 Signaling,Parkinson's Disease,Prolactin Signaling,Prostate Cancer,Signal Transduction,TNF Signaling,Toll-like Receptor Signaling,Translational Control,TWEAK Signaling,Viral Carcinogenesis
Research Areas (Faceted)
cancer,genetics,signal_transduction,neurobiology,cell_biology,signal_transduction,immunology,cardiovascular,signal_transduction,cell_biology,cell_cycle,genetics,transcription_translation,signal_transduction,developmental_biology,infectious_disease,neurobiology,immunology,signal_transduction,cell_biology,metabolism,neurobiology,developmental_biology,signal_transduction,neurobiology,immunology,genetics,transcription_translation,cancer,infectious_disease
Species
human
Target Sentence
Caspase 3 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Caspase 3 cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. Caspase 3 is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease [taken from NCBI Entrez Gene (Gene ID: 836)].
UniGene ID
Hs.141125
UniProt ID (eCommerce)
P42574

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.