human AKT3 shRNA Adenovirus

Name 
Ad-h-AKT3-shRNA
Cat No 
1803
Availability
Immediate

This is an pre-made gene silencing adenovirus that expresses a shRNA to knockdown human AKT3 gene. The shRNA expression is driven by an U6 promoter.

The knockdown of this human gene was validated by qPCR in A549 cells.

1803
Ad-h-AKT3-shRNA

Ready-to-use human AKT3 shRNA Adenovirus. human AKT3 shRNA adenovirus gene knockdown1803

$995.00
Request Volume Pricing or Customization

Product Details

Promoter
U6 ()
Reporter
eGFP
shRNA Knockdown (%)
85
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Volume (µL)
200

Gene Reference Data

Alternate Names
MPPH; MPPH2; PKB-GAMMA; PKBG; PRKBG; RAC-gamma; RAC-PK-gamma; STK-2
Description (Vector)
AKT3 is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described.
Gene ID
10000
Gene Name (Vector)
AKT serine/threonine kinase 3
Gene Symbol
AKT3
HGNC ID
HGNC:393
ORF Size (aa)
1398
ORF Size (bp)
1398 bp
RefSeq ID
NM_005465
RefSeq Synonyms
NM_181690, NM_005465, NM_001206729, BC121154,
Species
human
UniGene ID
Hs.498292

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.