codon-optimized FLP (FLPo) with RFP Adenovirus

Name 
Ad-RFP-FLPo
Cat No 
1777
Availability
Immediate
Categories
Adenovirus, Over-Expression, Recombinase

FLP is a site-specific recombinases (SSR) from the λ integrase family, which recognizes distinct 34 bp FRT sites. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe, with a 4-fold increase in recombination efficiency at 37C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin.

FLPo is a mouse codon optimized FLPe that greatly increases the FRT recombination efficiency in mouse cells. This adenovirus express FLPo, along with a RFP marker. FLPo and RFP are driven by the same CMV promoter and separated by a 2A peptide.

1777
Ad-RFP-FLPo

Ready-to-use codon-optimized FLP (FLPo) with RFP Adenovirus. 1777 FLPo FLP RFP adenovirus Ad-RFP-FLP Ad-FLP-RFP

$495.00
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Product Details

Promoter
CMV (ubiquitous)
Reporter
RFP
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Volume (µL)
200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.