codon optimized Cre (iCre) and GFP Adenovirus

Name 
Ad-GFP-2A-iCre
Cat No 
1772
Availability
Immediate

Cre Recombinase is a Type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites. loxP is a 34 bp DNA sequence at which confers directionality. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals.

By applying the mammalian codon usage to Cre recombinase, expression of Cre is improved in the mammalian cells. This improved Cre (iCre) gene also reduce the high CpG content of the prokaryotic coding sequence, thereby reducing the chances of epigenetic silencing in mammals.

This adenovirus expresses both a codon improved Cre recombinase (iCre) and eGFP as marker. Cre and GFP are driven by the same CMV promoter and separated by 2A peptides.

Ad-Cre Video
Check out this instruction video on using our adenovirus Cre to knockout LoxP flanked gene in primary mouse embryonic fibroblasts (MEF).

1772
Ad-GFP-2A-iCre

Ready-to-use codon optimized Cre (iCre) and GFP Adenovirus. Ad-Cre-GFP Cre GFP adenovirus 1772 Ad-GFP-iCre Ad-GFP-2A-Cre

$475.00
Request Volume Pricing or Customization

Product Details

Promoter
CMV (ubiquitous)
Reporter
eGFP
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Volume (µL)
200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.