codon optimized Cre (iCre) and GFP Adenovirus
Cre Recombinase is a Type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites. loxP is a 34 bp DNA sequence at which confers directionality. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals.
By applying the mammalian codon usage to Cre recombinase, expression of Cre is improved in the mammalian cells. This improved Cre (iCre) gene also reduce the high CpG content of the prokaryotic coding sequence, thereby reducing the chances of epigenetic silencing in mammals.
This adenovirus expresses both a codon improved Cre recombinase (iCre) and eGFP as marker. Cre and GFP are driven by the same CMV promoter and separated by 2A peptides.
Check out this instruction video on using our adenovirus Cre to knockout LoxP flanked gene in primary mouse embryonic fibroblasts (MEF).
Ready-to-use codon optimized Cre (iCre) and GFP Adenovirus. Ad-Cre-GFP Cre GFP adenovirus 1772 Ad-GFP-iCre Ad-GFP-2A-Cre
Related Citations
- Selective role of Nck1 in atherogenic inflammation and plaque formation. AW Orr, etc, (2020), JCI
- Lens fiber cell differentiation occurs independently of fibroblast growth factor receptor signaling in the absence of Pten. SL Padula, etc, (2020), Developmental Biology
- An Ovol2-Zeb1 transcriptional circuit regulates epithelial directional migration and proliferation. D Haensel, etc, (2018), EMBO reports
- N-terminal Huntingtin Knock-In Mice: Implications of Removing the N-terminal Region of Huntingtin for Therapy. Xudong Liu, etc, (2016), PLoS Genetics
- L-type voltage-operated calcium channels contribute to astrocyte activation In vitro. VT Cheli, etc, (2016), GLIA
- The HIF-1/glial ¿TIM-3 axis controls inflammation-associated brain damage under hypoxia. Han Seok Koh, etc, (2015), Nature Communications