Cre and RFP expressing RGD fiber modified Adenovirus

Name 
Ad(RGD)-RFP-iCre
Cat No 
1770
Availability
Immediate
Categories
Adenovirus, Fiber-modified Ad5, Over-Expression

Since the cell entry of adenovirus is dependent on the initial recognition of the coxsackie-adenovirus receptor (CAR) on the surafce, the infection efficiency of adenovirus is very low for cells with low or no CAR receptor. An Arg-Gly-Asp (RGD) motif was introduced in the surface-exposed loop of the adenovirus fiber knob, this enables the adenovirus to bypass CAR and mediate cell entry via RGD binding integrins.

RDG modified adenovirus can be used for “retargeting” cells that regular adenovirus doesn’t work well. The infection effeciency of RDG modified adenovirus can be tens or hundreds of times higher than standard Ad5 in some cells, including human or mouse macrophages and T cells etc.

This is a RGD modified replication-deficient adenovirus expressing codon optimized Cre and RFP under a CMV promoter. The Cre and RFP are separated by a 2A peptide.

1770
Ad(RGD)-RFP-iCre

Ready-to-use Cre and RFP expressing RGD fiber modified Adenovirus. 1770 Cre Ad(RGD)-RFP-2A-iCre RFP adenovirus RGD

$495.00
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Product Details

Promoter
CMV (ubiquitous)
Reporter
RFP
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3) with RGD-fiber modification
Volume (µL)
200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.