Chk1 Checkpoint homolog (S. pombe) Adenovirus

Name: Chk1 Checkpoint homolog (S. pombe) Adenovirus
SKU: 1593
Availability: InStock

Name
Ad-CHK1/CHEK1

Cat No
1593

Availability
Immediate

DNA damage or incomplete replication of DNA results in the inhibition of cell cycle progression at the G1 to S or the G2 to M phase transition by conserved regulatory mechanisms known as cell cycle checkpoints. Checkpoint proteins include Rad17, which is involved in regulating cell cycle progression at the G1 checkpoint, and Chk1, Chk2, Rad1, Rad9 and Hus1, which are involved in regulating cell cycle arrest at the G2 checkpoint. Overexpression of Rad17 results in the activation of p53 and an accumulation of cells in G1. Bub1 is involved in cell cycle checkpoint enforcement while Bub2 is part of a checkpoint that prevents premature exit from mitosis and monitors spindle integrity.

1593

Ad-CHK1/CHEK1

Ready-to-use Chk1 Checkpoint homolog (S. pombe) Adenovirus. Ad-CHK1/CHEK1, Chk1 Checkpoint homolog (S. pombe), CHK1, CHEK1, CHK-1 adenovirus 1593

$690.00

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Product Details

Promoter

CMV (ubiquitous)

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Titer

1x10^10 PFU/ml

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Volume (µL)

200

Gene Reference Data

Alternate Names

cell cycle checkpoint kinase; Checkpoint kinase-1; Checkpoint, S. pombe, homolog of, 1; CHK1; CHK1 checkpoint homolog; Chk1-S; serine/threonine-protein kinase Chk1

Description (eCommerce)

Chk1 functions as a checkpoint protein kinase that is critical to the regulation of the Cdc25a and Cdc25c phosphatases that promote S and G2/M phase progression, respectively. Chk1 kinase activity inhibits cell cycle progression in the presence of DNA damage by phosphorylating Cdc25a and Cdc25c and causing the degradation of Cdc25a and cytoplasmic sequestration of Cdc25c.

Description (Vector)

CHEK1 belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.

Gene ID

1111

Gene Name (eCommerce)

checkpoint kinase 1

Gene Name (Vector)

checkpoint kinase 1

Gene Symbol

CHEK1

HGNC ID

HGNC:1925

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

1431

ORF Size (bp)

1431 bp

Protein Name (eCommerce)

Serine/threonine-protein kinase Chk1

RefSeq ID

NM_001274

RefSeq Synonyms

NM_001330428, NM_001330427, NM_001274, NM_001244846, NM_001114122, NM_001114121, BC017575, BC004202,

Research Areas

Breast Cancer,Cancer,Cell Cycle,Cytoskeleton,DNA Damage/Repair,DNA Replication,Fanconi anemia,Histone Modification,Homologous Recombination,Host-Virus Interactions,Kinase/Phosphatase,p53 Signaling,Phospho-specific,Prostate Cancer,Replication,SCF-KIT Signaling,Signal Transduction,Tumor Suppressors/Oncoproteins,Viral Carcinogenesis

Research Areas (Faceted)

cancer,cell_biology,cell_cycle,genetics,transcription_translation,infectious_disease,signal_transduction,stem_cell_biology

Species

human

Target Sentence

UniGene ID

Hs.24529

UniProt ID (eCommerce)

O14757

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.