Zeta-chain (TCR) associated protein kinase 70kDa Adenovirus

Name 
Ad-ZAP-70
Cat No 
1423
Availability
Immediate
Categories
Adenovirus, Over-Expression

Syk p72 and ZAP-70 resemble the Src family protein tyrosine kinases in that they have a C-terminal catalytic domain, but differ in that they are characterized by two SH2 domains but no SH3 domains. Syk p72 kinase fused to the transmembrane and extracellular domains of CD7 and CD16, respectively, can induce complete T cell activation, implying the involvement of the Syk/ZAP family proteins in T cell activation. In contrast, the ZAP-70 kinase is insufficient for complete T cell activation unless it is coaggregated with a Fyn p59-containing chimera, suggesting that regulation of ZAP-70 activity may require a functional interaction with Src family kinases.

1423
Ad-ZAP-70

Ready-to-use Zeta-chain (TCR) associated protein kinase 70kDa Adenovirus. Ad-ZAP-70, Zeta-chain (TCR) associated protein kinase 70kDa, SRK,STD,TZK,ZAP70,ZAP-70 adenovirus 1423

$690.00
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Product Details

Promoter
CMV (ubiquitous)
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Volume (µL)
200

Gene Reference Data

Alternate Names
70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; IMD48; SRK; STCD; STD; syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; TZK; ZAP-70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
Description (eCommerce)
ZAP-70 (zeta associated protein 70) is a tyrosine kinase of the Src family of tyrosine kinases. It was initially identified as a partner of the zeta chain of the CD3 complex associated to the T-cell receptor on T-lymphocytes. ZAP-70 shares homology with spleen-tyrosine kinase (Syk), a kinase involved in B-cell activation. Both ZAP-70 and Syk have been shown to be crucial to B- and T-lymphocyte development. In T-cells ZAP-70 functions to transduce the signaling cascade activated by T-cell receptor engagement and activation.
Description (Vector)
ZAP70 encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
Gene ID
7535
Gene Name (eCommerce)
zeta chain of T cell receptor associated protein kinase 70
Gene Name (Vector)
zeta chain of T cell receptor associated protein kinase 70
Gene Symbol
ZAP70
HGNC ID
HGNC:12858
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1482
ORF Size (bp)
1482 bp
Protein Name (eCommerce)
Tyrosine-protein kinase ZAP-70
RefSeq ID
NM_001079
RefSeq Synonyms
NM_207519, NM_001079, BC053878, BC039039,
Research Areas
Apoptosis,B-cell Receptor Signaling,EPO Signaling,IFN Signaling,Immunology,Interleukin Signaling,JAK-STAT Signaling,Kinase/Phosphatase,Neurobiology,NF-kB Signaling,Phospho-specific,Prolactin Signaling,Signal Transduction,T-cell Receptor Signaling,VEGF Signaling
Research Areas (Faceted)
signal_transduction,cell_biology,immunology,signal_transduction,cell_biology,neurobiology,signal_transduction,immunology,cell_biology,signal_transduction,immunology
Species
human
Target Sentence
ZAP-70 (zeta associated protein 70) is a tyrosine kinase of the Src family of tyrosine kinases. It was initially identified as a partner of the zeta chain of the CD3 complex associated to the T-cell receptor on T-lymphocytes. ZAP-70 shares homology with spleen-tyrosine kinase (Syk), a kinase involved in B-cell activation. Both ZAP-70 and Syk have been shown to be crucial to B- and T-lymphocyte development. In T-cells ZAP-70 functions to transduce the signaling cascade activated by T-cell receptor engagement and activation.
UniGene ID
Hs.234569
UniProt ID (eCommerce)
P43403

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.