p16/CDKN2A Adenovirus

Name 
Ad-CMV-p16
Cat No 
1040
Availability
Immediate
Categories
Adenovirus, Over-Expression

p16/CDKN2A is a cyclin-dependent kinase inhibitor that has shown prognostic utility in some human cancers. p16 is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.

p16/CDKN2A generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame(ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control.

This adenovirus expresses cyclin-dependent kinase inhibitor 2A (CDKN2A) isoform p16INK4a, with the first 8 amino acid deleted from the N-terminal.

1040
Ad-CMV-p16

Ready-to-use p16/CDKN2A Adenovirus. p16 CDKN2A adenovirus 1040

$475.00
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Product Details

Promoter
CMV (ubiquitous)
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Volume (µL)
200

Gene Reference Data

Alternate Names
alternative reading frame;ARF;CDK4 inhibitor p16-INK4;CDK4I;CDKN2;cell cycle negative regulator beta;CMM2;cyclin-dependent kinase 4 inhibitor A;cyclin-dependent kinase inhibitor 2A;cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4);INK4;INK4A;MLM;MTS1;MTS-1;multiple tumor suppressor 1;P14;P14ARF;P16;P16INK4;p16-INK4;P16INK4A;P16-INK4A;P19;P19ARF;TP16
Description (eCommerce)
p16INK4a is a negative regulator of CDK4 and CDK6 kinase activity and cell cycle progression. p16INK4a regulates the cell cycle by inhibiting cyclin/CDK complex formation and the phosphorylation of the retinoblastoma protein (pRb). The gene which encodes p16INK4a, CDKN2A, is frequently mutated or deleted in a wide variety of tumors and is known to be an important tumor suppressor gene. The CDKN2A gene also generates a distinct transcript variant and protein, p14ARF, from an alternate open reading frame (ARF).
Gene ID
1029
Gene Name (eCommerce)
cyclin dependent kinase inhibitor 2A
Gene Symbol
CDKN2A
NCBI Taxonomy ID (eCommerce)
9606.0
Protein Name (eCommerce)
Cyclin-dependent kinase inhibitor 2A
RefSeq ID
NM_000077
RefSeq Synonyms
NM_058197, NM_058195, NM_001195132, NM_000077,
Research Areas
Aging,Apoptosis,Autophagy,Bladder Cancer,Cancer,Cell Cycle,DNA Damage/Repair,Glioma,Hedgehog Signaling,Host-Virus Interactions,Hypoxia,Immunology,Leukemia,Lung Cancer,MicroRNAs in Cancer,Mitochondrion,Neurobiology,p53 Signaling,RNA Processing,Signal Transduction,Transcription Factor / Regulator,Tumor Suppressors/Oncoproteins,Viral Carcinogenesis
Research Areas (Faceted)
cancer,cell_biology,cell_cycle,genetics,immunology,infectious_disease,neurobiology,signal_transduction,transcription_translation
Species
human
Target Sentence
p16INK4a is a negative regulator of CDK4 and CDK6 kinase activity and cell cycle progression. p16INK4a regulates the cell cycle by inhibiting cyclin/CDK complex formation and the phosphorylation of the retinoblastoma protein (pRb). The gene which encodes p16INK4a, CDKN2A, is frequently mutated or deleted in a wide variety of tumors and is known to be an important tumor suppressor gene. The CDKN2A gene also generates a distinct transcript variant and protein, p14ARF, from an alternate open reading frame (ARF).
UniProt ID (eCommerce)
Q8N726

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.