Ras (N17) Adenovirus
Cat. No: 1031
Name: Ad-CMV-Ras (N17)
Ras genes encode 21 kDa guanine nucleotide-binding proteins, including H-, K- and N-Ras. H-Ras was first identified as oncogene, mutated Ras genes have been found in many human tumors. Like all GTPases, Ras act as molecular switch to control downstream cellular events. The interconversion of the inactive GDP-bound form into the active GTP-bound form is regulated by guanine nucleotide exchange factors, whereas inactivation of the GTP-bound form is stimulated by GTPase-activating proteins (GAPs). Ras in its active GTP bound form binds to Raf, resulting in activation of MAP kinase cascade. The provided recombinant adenovirus contains dominant negative form of human H-Ras (T17N).
Request a Quote
Please enter your email address and we'll be in touch with more information:
- Viral Backbone
- Human Adenovirus Type5 (dE1/E3)
- CMV (ubiquitous)
- Storage Buffer
- DMEM, 2% BSA, 2.5% Glycerol
- 1x10^10 PFU/ml
This product is referenced in the following publications:
- Wu Feng R., etc. (2007). Hiv-1 Tat Activates Dual Nox Pathways Leading To Independent Activation Of Erk And Jnk Map Kinases. J Biol Chem.
- Zhang, T. etc (2011). Mitogen-activated protein kinase phosphatase-1 inhibits myocardial TNF-alpha expression and improves cardiac function during endotoxemia. Cardiovascular Research.
- Rounak Nande, etc (2012). Targeting a Newly Established Spontaneous Feline Fibrosarcoma Cell Line by Gene Transfer. PLoS ONE.
- Nagalingam, RS. etc (2013). A cardiac enriched microRNA, miR-378 blocks cardiac hypertrophy by targeting Ras-signaling. J Biol Chem.
- RS Nagalingam, etc (2014). Deficiency of Cardiomyocyte-specific-microRNA-378 Contributes to the Development of Cardiac Fibrosis involving a TGFß1-dependent Paracrine Mechanism. JBC.
- Ma Z, etc (2010). p66Shc restrains Ras hyperactivation and suppresses metastatic behavior. Oncogene.