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mouse CHST4 Adenovirus

Name 
Ad-m-CHST4
Cat No 
ADV-255479
Availability
4-5 weeks
Categories
Adenovirus, Over-Expression

This is an Adenovirus expressing Mouse CHST4.

ADV-255479
Ad-m-CHST4

Ready-to-use Adenovirus expressing Mouse Chst4 (carbohydrate (chondroitin 6/keratan) sulfotransferase 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
CMV
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
Gn6st-2; GST-3; HEC-GlcNAc6ST
Description (Vector)
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X on receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2-branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location.
Gene ID
26887
Gene Name (Vector)
carbohydrate (chondroitin 6/keratan) sulfotransferase 4
Gene Symbol
Chst4
ORF Size (aa)
1167
ORF Size (bp)
1167 bp
RefSeq ID
NM_011998
RefSeq Synonyms
NM_011998, BC057886,
Species
mouse
UniGene ID
Mm.89207

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.