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human TOP2A Adenovirus

Name 
Ad-h-TOP2A
Cat No 
ADV-226203
Availability
4-5 weeks
Categories
Adenovirus, Over-Expression

This is an Adenovirus expressing Human TOP2A.

ADV-226203
Ad-h-TOP2A

Ready-to-use Adenovirus expressing Human TOP2A (topoisomerase (DNA) II alpha 170kDa). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
CMV
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
DNA gyrase; DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase 2-alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; TOP2; topoisomerase (DNA) II alpha 170kDa; TP2A
Description (eCommerce)
Topoisomerases regulate topological states of DNA and are divided into type I and type II enzymes. Type II topoisomerases make double stranded breaks in DNA and allow the passage of another duplex DNA across the break to achieve unknotting of entangled DNA. Topo II Alpha is one of two described isoforms that are likely products of a gene duplication event. TopoII Alpha is also described to be involved in chromosome condensation, chromatid separation, and the relief of supercoil stress.
Description (Vector)
TOP2A encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.
Gene ID
7153
Gene Name (eCommerce)
DNA topoisomerase II alpha
Gene Name (Vector)
topoisomerase (DNA) II alpha 170kDa
Gene Symbol
TOP2A
HGNC ID
HGNC:11989
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
4596
ORF Size (bp)
4596 bp
Protein Name (eCommerce)
DNA topoisomerase 2-alpha
RefSeq ID
NM_001067
RefSeq Synonyms
NM_001067, BC140791,
Research Areas
Aging,Apoptosis,Cancer,Cell Cycle,Chromatin Remodeling,DNA Damage/Repair,DNA Replication,Histone Modification,Host-Virus Interactions,Signal Transduction,Ubiquitination
Research Areas (Faceted)
cancer,signal_transduction,cell_biology,cell_cycle,genetics,transcription_translation,infectious_disease,genetics
Species
human
Target Sentence
Topoisomerases regulate topological states of DNA and are divided into type I and type II enzymes. Type II topoisomerases make double stranded breaks in DNA and allow the passage of another duplex DNA across the break to achieve unknotting of entangled DNA. Topo II Alpha is one of two described isoforms that are likely products of a gene duplication event. TopoII Alpha is also described to be involved in chromosome condensation, chromatid separation, and the relief of supercoil stress.
UniGene ID
Hs.156346
UniProt ID (eCommerce)
P11388

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.