human APOA1 Over-expression AAV

Name 
AAV-h-APOA1
Cat No 
AAV-201161
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human APOA1.

AAV-201161
AAV-h-APOA1

Ready-to-use AAV expressing Human APOA1 (apolipoprotein A1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
apo(a); apo-AI; ApoA-I; Apolipoprotein A1; apolipoprotein A-I; epididymis secretory sperm binding protein
Description (eCommerce)
Apo AI comprises approximately 70% of the protein moiety in HDL. It is a single polypeptide chain consisting of 243 amino acid residues without disulfide bound and with glutamic acid as the C-terminal residue and aspartic acid as the N-terminal residue. The molecular weight is reported to be 28 kDa (Brewer et al., 1978). The roles of Apo AI in HDL function include reverse cholesterol transportation, lipid cholesterol binding, lecithin-cholesterol acyl transferase (LCAT) activation, and receptor binding, which is responsible for cholesterol esterification in plasma. Besides participate in cholesterol metabolism, Apo AI and HDL also suppress neutrophil activation, inhibit bacterial endotoxin, induce trypanosomal lysis, and other physiological activities. (Brouillette et al., 2001). Apo AI levels may be inversely related to the risk of coronary disease. In previous research, Apo AI may affect diet-induced inflammation by either directly or indirectly altering lipid rafts. (Cheng et al., 2012)
Description (Vector)
APOA1 encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. APOA1 is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis.
Gene ID
335
Gene Name (eCommerce)
apolipoprotein A1
Gene Name (Vector)
apolipoprotein A1
Gene Symbol
APOA1
HGNC ID
HGNC:600
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
804
ORF Size (bp)
804 bp
Protein Name (eCommerce)
Apolipoprotein A-I
RefSeq ID
NM_000039
RefSeq Synonyms
NM_001318021, NM_001318018, NM_001318017, NM_000039, BC110286, BC005380,
Research Areas
Cardiovascular
Research Areas (Faceted)
cardiovascular
Species
human
Target Sentence
Apo AI comprises approximately 70% of the protein moiety in HDL. It is a single polypeptide chain consisting of 243 amino acid residues without disulfide bound and with glutamic acid as the C-terminal residue and aspartic acid as the N-terminal residue. The molecular weight is reported to be 28 kDa (Brewer et al., 1978). The roles of Apo AI in HDL function include reverse cholesterol transportation, lipid cholesterol binding, lecithin-cholesterol acyl transferase (LCAT) activation, and receptor binding, which is responsible for cholesterol esterification in plasma. Besides participate in cholesterol metabolism, Apo AI and HDL also suppress neutrophil activation, inhibit bacterial endotoxin, induce trypanosomal lysis, and other physiological activities. (Brouillette et al., 2001). Apo AI levels may be inversely related to the risk of coronary disease. In previous research, Apo AI may affect diet-induced inflammation by either directly or indirectly altering lipid rafts. (Cheng et al., 2012)
UniGene ID
Hs.633003
UniProt ID (eCommerce)
P02647

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.