CRISPR/Cas9 nuclease with FLAG tag Adenovirus
The Type II prokaryotic CRISPR/Cas system is the new class of tools for targeted genome engineering. The cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-cas systems uses small RNAs as guides (gRNA) to cleave DNA in a sequence-specific manner. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms.
For site-specific genome editing, the CRISPR/Cas9 system requires the Cas 9 nuclease and the gRNA. This adenovirus expresses a codon-optimized Cas9 nuclease with a N-terminal FLAG tag and a GFP reporter. It can be used along with guided RNA for genome-editing in cells.
Ready-to-use CRISPR/Cas9 nuclease with FLAG tag Adenovirus. CRISPR Cas9 adenovirus Ad-GFP-FLAG-Cas9 Ad-GFP-Cas9 1904
Related Citations
- Targeting nuclear pore protein, NUP210, reduces metastasis through heterochromatinmediated silencing of mechanosensitive genes. R Amin, etc, (2020), bioRxiv
- In vivo genome and base editing of a human PCSK9 knock-in hypercholesterolemic mouse model. A Carreras, etc, (2019), BMC Biology
- In vivo CRISPR editing with no detectable genome-wide off-target mutations. P Akcakaya, etc, (2018), Nature
- Therapeutic Genome Editing With CRISPR/Cas9 in a Humanized Mouse Model Ameliorates a1-antitrypsin Deficiency Phenotype. M Bjursell, etc, (2018), EBioMedicine