human NOTCH1 shRNA Adenovirus

Name 
Ad-h-NOTCH1-shRNA
Cat No 
1853
Availability
Immediate
Categories
Adenovirus, shRNA-Silencing

This is an pre-made gene silencing adenovirus that expresses a shRNA to knockdown human NOTCH 1 gene. The shRNA expression is driven by an U6 promoter.

The knockdown of this human gene was validated by western blot in A549 cells.

1853
Ad-h-NOTCH1-shRNA

Ready-to-use human NOTCH1 shRNA Adenovirus. human NOTCH1 shRNA adenovirus gene knockdown1853

$995.00
Request Volume Pricing or Customization

Product Details

Promoter
U6 ()
Reporter
eGFP
shRNA Knockdown (%)
75
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Volume
200 µL

Gene Reference Data

Alternate Names
AOS5; AOVD1; hN1; neurogenic locus notch homolog protein 1; Notch 1; Notch homolog 1, translocation-associated; TAN1; translocation-associated notch protein TAN-1
Description (eCommerce)
Notch1 is the human homolog of the D. melanogaster Notch gene first reported as the gene responsible for the presence of notches in the wingblades of a specific strain of flies. The Notch protein is a transmembrane receptor that mediates juxtacrine signals between adjacent cells. Mammalian Notch1 ligands include Delta-like and Jagged. Upon ligand binding, Notch1 undergoes two proteolytic cleavage events that result in the release of the intracellular Notch1 domain and its translocation to the nucleus where it can participate in gene transactivation. Notch 1 signals mediate cellular fate, differentiation, and development.
Gene ID
4851
Gene Name (eCommerce)
notch 1
Gene Symbol
NOTCH 1
NCBI Taxonomy ID (eCommerce)
9606.0
Protein Name (eCommerce)
Neurogenic locus notch homolog protein 1
RefSeq ID
NM_017617
RefSeq Synonyms
NM_017617,
Research Areas
Alzheimer's Disease,Angiogenesis,Cancer,Cardiac Development,Cardiology,Cardiovascular,Gene Expression,Golgi Apparatus,Immunology,JAK-STAT Signaling,MicroRNAs in Cancer,Neurobiology,Neurodegeneration,Neurodevelopment,Neurogenesis,Notch Signaling,RNA Binding,Signal Transduction,Spermatogenesis,Transcription Factor/Regulator,Wnt Signaling
Research Areas (Faceted)
cancer,genetics,signal_transduction,neurobiology,developmental_biology,cardiovascular,genetics,transcription_translation,cell_biology,immunology,signal_transduction,neurobiology,developmental_biology,signal_transduction,genetics,cell_biology,cell_cycle,genetics,transcription_translation,signal_transduction,developmental_biology
Species
human
Target Sentence
Notch1 is the human homolog of the D. melanogaster Notch gene first reported as the gene responsible for the presence of notches in the wingblades of a specific strain of flies. The Notch protein is a transmembrane receptor that mediates juxtacrine signals between adjacent cells. Mammalian Notch1 ligands include Delta-like and Jagged. Upon ligand binding, Notch1 undergoes two proteolytic cleavage events that result in the release of the intracellular Notch1 domain and its translocation to the nucleus where it can participate in gene transactivation. Notch 1 signals mediate cellular fate, differentiation, and development.
UniProt ID (eCommerce)
P46531

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.