human mTOR shRNA Adenovirus

Name 
Ad-h-mTOR-shRNA
Cat No 
1852
Availability
Immediate
Categories
Adenovirus, shRNA-Silencing

This is an pre-made gene silencing adenovirus that expresses a shRNA to knockdown human mTOR gene. The shRNA expression is driven by an U6 promoter.

The knockdown of this human gene was validated by western blot in A549 cells.

1852
Ad-h-mTOR-shRNA

Ready-to-use human mTOR shRNA Adenovirus. human mTOR shRNA adenovirus gene knockdown1852

$995.00
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Product Details

Promoter
U6 ()
Reporter
eGFP
shRNA Knockdown (%)
70
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Volume
200 µL

Gene Reference Data

Alternate Names
FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FRAP; FRAP1; FRAP2; mammalian target of rapamycin; Mechanistic target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mTOR; RAFT1; rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; rapamycin target protein 1; RAPT1; serine/threonine-protein kinase mTOR; SKS
Description (eCommerce)
Studies of the mammalian target of rapamycin (mTOR) and its homologs demonstrate a role in integrating signals from growth factors, nutrients, stress, and cellular energy levels to control cell growth, translation initiation, ribosome biogenesis, and transcription factor localization. mTOR is the direct target of the cell cycle arresting activity of rapamycin. mTOR interacts with Raptor or Rictor to form the mTORC1 and mTORC2 complexes respectively. The mTORC1 complex also includes mLst8/GbetaL and functions to phosphorylate S6K and 4EBP1. The mTORC2 complex also includes mLst8/GbetaL, mSIN1 and protor-1 and functions to phosphorylate Akt.
Description (Vector)
MTOR belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.
Gene ID
2475
Gene Name (eCommerce)
mechanistic target of rapamycin kinase
Gene Name (Vector)
mechanistic target of rapamycin
Gene Symbol
MTOR
HGNC ID
HGNC:3942
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
7650
ORF Size (bp)
7650 bp
Protein Name (eCommerce)
Serine/threonine-protein kinase mTOR
RefSeq ID
NM_004958
RefSeq Synonyms
NM_004958, BC117166,
Research Areas
Aging,Angiogenesis,Autophagy,B-cell Receptor Signaling,BDNF Signaling,Breast Cancer,Cancer,Cardiac Hypertrophy,Cardiology,Cardiovascular,Cytoskeleton,EGFR Signaling,EGFR Signaling in Cancer,EPO Signaling,Fc Receptor Signaling,FGF Signaling,Glioma,Golgi Apparatus,HGF Signaling,HIF-1 Signaling,Hypoxia,IFN Signaling,IGF-1 Signaling,Immunology,Innate Immunity,Insulin Signaling,Interleukin Signaling,JAK-STAT Signaling,Kinase/Phosphatase,Leptin Signaling,Leukemia,LKB1 Signaling,Lung Cancer,Lysosome,MicroRNAs in Cancer,Mitochondrion,mTOR Signaling,Neurobiology,NGF Signaling,PDGF Signaling,PI3K/Akt Signaling,PI3K-AKT Signaling in Cancer,Prolactin Signaling,Prostate Cancer,RANKL-RANK Signaling,RNA Binding,SCF-KIT Signaling,Signal Transduction,Transcription Factor/Regulator,Translational Control,TSH Signaling,TSLP Signaling,VEGF Signaling,Wnt Signaling
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,developmental_biology,genetics,immunology,neurobiology,signal_transduction,stem_cell_biology,transcription_translation
Species
human
Target Sentence
Studies of the mammalian target of rapamycin (mTOR) and its homologs demonstrate a role in integrating signals from growth factors, nutrients, stress, and cellular energy levels to control cell growth, translation initiation, ribosome biogenesis, and transcription factor localization. mTOR is the direct target of the cell cycle arresting activity of rapamycin. mTOR interacts with Raptor or Rictor to form the mTORC1 and mTORC2 complexes respectively. The mTORC1 complex also includes mLst8/GbetaL and functions to phosphorylate S6K and 4EBP1. The mTORC2 complex also includes mLst8/GbetaL, mSIN1 and protor-1 and functions to phosphorylate Akt.
UniGene ID
Hs.338207
UniProt ID (eCommerce)
P42345

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.