human Oct-4 Adenovirus

Name 
Ad-h-Oct4
Cat No 
1785
Availability
Immediate
Categories
Adenovirus, iPS stem cell factors, Over-Expression

Oct-3/4 is one of the family of octamer (“Oct”) transcription factors, and plays a crucial role in maintaining pluripotency. The absence of Oct-3/4 in Oct-3/4+ cells, such as blastomeres and embryonic stem cells, leads to spontaneous trophoblast differentiation, and presence of Oct-3/4 thus gives rise to the pluripotency and differentiation potential of embryonic stem cells. Various other genes in the “Oct” family, including Oct-3/4’s close relatives, Oct1 and Oct6, fail to elicit induction, thus demonstrating the exclusiveness of Oct-3/4 to the induction process.

Co-expression of Oct4, Sox2, KLF4 and c-Myc (Yamanaka set) in somatic cell will lead to iPSC reprogramming. Using adenovirus to deliver these transcription factors has 2 main advantages – it does not incorporate into the targeted host and therefore avoids the potential for insertional mutagenesis, and the expression is transient for a brief amount of time in order for effective reprogramming to take place.

This adenovirus expresses human Oct4, and it can be used in combination with other iPSC factor for the induction of iPSC reprogramming.

1785
Ad-h-Oct4

Ready-to-use human Oct-4 Adenovirus. Oct4 Oct-4 adenovirus iPS POU5F1 OCT3 1785

$475.00
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Product Details

Promoter
CMV (ubiquitous)
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Volume (µL)
200

Gene Reference Data

Alternate Names
Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4
Description (Vector)
POU5F1 encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
Gene ID
5460
Gene Name (Vector)
POU class 5 homeobox 1
Gene Symbol
POU5F1
HGNC ID
HGNC:9221
ORF Size (aa)
1083
ORF Size (bp)
1083 bp
RefSeq ID
NM_002701
RefSeq Synonyms
NM_203289, NM_002701, NM_001285987, NM_001285986, NM_001173531, BC117437, BC117435,
Species
human
UniGene ID
Hs.249184

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.