WEE1 homolog Adenovirus

Name 
Ad-WEE1
Cat No 
1459
Availability
Immediate
Categories
Adenovirus, Over-Expression

Phosphorylation of Cdc2 on threonine 14 and tyrosine 15 is required to maintain Cdc2 in an inactive state throughout the S and G2 phases of the cell cycle. The human Wee 1 protein, Wee 1 Hu, encodes a tyrosine-specific protein kinase that phosphorylates Cdc2 on tyrosine 15. Myt 1, a member of the Wee 1 family of protein kinases, has been shown to phosphorylate Cdc2 on both threonine 14 and tyrosine 15 in a cyclin-dependent manner. Both Wee 1 Hu and Myt 1 activity are regulated during the cell cycle, suggesting that both proteins play a role in mitotic control. Dephosphorylation of Cdc2 on threonine 14 and tyrosine 15 in late G2 by Cdc25 then activates the Cdc2/cyclin B complex to allow entry into mitosis.

1459
Ad-WEE1

Ready-to-use WEE1 homolog Adenovirus. Ad-WEE1, WEE1 homolog, WEE1,WEE1HU adenovirus 1459

$690.00
Request Volume Pricing or Customization

Product Details

Promoter
CMV (ubiquitous)
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Titer
1x10^10 PFU/ml
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Volume
200 µL

Gene Reference Data

Alternate Names
WEE1A; WEE1hu
Description (Vector)
WEE1 encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
Gene ID
7465
Gene Name (Vector)
WEE1 G2 checkpoint kinase
Gene Symbol
WEE1
HGNC ID
HGNC:12761
ORF Size (aa)
1941
ORF Size (bp)
1941 bp
RefSeq ID
NM_003390
RefSeq Synonyms
NM_003390, NM_001143976, BC070052, BC051831,
Species
human
UniGene ID
Hs.249441

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.