human CLSPN Adenovirus

Name 
Ad-h-CLSPN
Cat No 
ADV-205510
Availability
4-5 weeks
Categories
Adenovirus, Over-Expression

This is an Adenovirus expressing Human CACTIN.

ADV-205510
Ad-h-CLSPN

Ready-to-use Adenovirus expressing Human CLSPN (claspin). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

Promoter
CMV
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
claspin; claspin homolog; hClaspin
Description (eCommerce)
Claspin is a checkpoint protein that interacts with and regulates checkpoint kinase 1 (Chk1) in response to replication stress. The interaction between claspin and Chk1 has been demonstrated to be required for ATR-dependent Chk1 activation. The interaction involves the phosphorylation of claspin that may be accomplished by Chk1 kinase activity. Claspin has been found to localize to chromatin in both the presence and absence of damaged DNA and to associate with PCNA, as an essential component of the DNA replication machinery. This association with the replication machinery may indicate a role for claspin in linking replication to DNA damage signaling and Chk1 activation.
Description (Vector)
The product of this gene is an essential upstream regulator of checkpoint kinase 1 and triggers a checkpoint arrest of the cell cycle in response to replicative stress or DNA damage. The protein is also required for efficient DNA replication during a normal S phase. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
63967
Gene Name (eCommerce)
claspin
Gene Name (Vector)
claspin
Gene Symbol
CLSPN
HGNC ID
HGNC:19715
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3828
ORF Size (bp)
3828 bp
Protein Name (eCommerce)
Claspin
RefSeq ID
NM_022111
RefSeq Synonyms
NM_022111, NM_001330490, NM_001190481, BC140789, BC137279, BC115026, BC115025, BC113116,
Research Areas
Apoptosis,Cell Cycle,DNA Damage/Repair,DNA Replication,Homologous Recombination,PLK1 Signaling,Replication,Signal Transduction
Research Areas (Faceted)
signal_transduction,cell_biology,cell_cycle,genetics,transcription_translation,signal_transduction,cell_biology,cell_cycle
Species
human
Target Sentence
Claspin is a checkpoint protein that interacts with and regulates checkpoint kinase 1 (Chk1) in response to replication stress. The interaction between claspin and Chk1 has been demonstrated to be required for ATR-dependent Chk1 activation. The interaction involves the phosphorylation of claspin that may be accomplished by Chk1 kinase activity. Claspin has been found to localize to chromatin in both the presence and absence of damaged DNA and to associate with PCNA, as an essential component of the DNA replication machinery. This association with the replication machinery may indicate a role for claspin in linking replication to DNA damage signaling and Chk1 activation.
UniGene ID
Hs.175613
UniProt ID (eCommerce)
Q9HAW4

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.