AAV expressing human pri-miR hsa-mir-221

Name 
AAV-EF1a-hsa-mir-221-eGFP
Cat No 
AAV-MI0000298
Availability
4-5 weeks
Categories
AAV, miRNA, Over-Expression

This is an AAV expressing Human pre-miRNA hsa-mir-221 under an EF1a promoter. This virus also has an eGFP as reporter.

We can also make this miRNA AAV with a different promoter (ubiquitous or cell-specific) or a fluorescence reporter other than eGFP. To learn more, simply request a quote with the details of your request.

AAV-MI0000298
AAV-EF1a-hsa-mir-221-eGFP

Ready-to-use AAV expressing Human pre-miRNA hsa-mir-221 under EF1a promoter with eGFP reporter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype
Choose from AAV 1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, DJ8, DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
EF1a
Reporter
eGFP
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV
Volume
200 µL

miRNA Reference Data

Label (English)
hsa-mir-221
Mature miRNA 1 Accession
MIMAT0004568
Mature miRNA 1 ID
hsa-miR-221-5p
Mature miRNA 1 Sequence
ACCUGGCAUACAAUGUAGAUUU
Mature miRNA 2 Accession
MIMAT0000278
Mature miRNA 2 ID
hsa-miR-221-3p
Mature miRNA 2 Sequence
AGCUACAUUGUCUGCUGGGUUUC
Pre-miRNA Accession
MI0000298
Pre-miRNA ID
hsa-mir-221
Pre-miRNA Sequence
UGAACAUCCAGGUCUGGGGCAUGAACCUGGCAUACAAUGUAGAUUUCUGUGUUCGUUAGGCAACAGCUACAUUGUCUGCUGGGUUUCAGGCUACCUGGAAACAUGUUCUC
Reference Entity Identifier
mirna
RefSeq ID
MI0000298

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.