mouse EIF3F Over-expression AAV

Name 
AAV-m-EIF3F
Cat No 
AAV-258209
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Mouse Eif3f.

AAV-258209
AAV-m-EIF3F

Ready-to-use AAV expressing Mouse Eif3f (eukaryotic translation initiation factor 3, subunit F). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
0610037M02Rik; AA409853; Eif3s5
Description (Vector)
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation|Function: Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling.
Gene ID
66085
Gene Name (Vector)
eukaryotic translation initiation factor 3, subunit F
Gene Symbol
Eif3f
ORF Size (aa)
1083
ORF Size (bp)
1083 bp
RefSeq ID
NM_025344
RefSeq Synonyms
NM_025344, BC083190, BC070473,
Species
mouse
UniGene ID
Mm.182962

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.