mouse CHMP4B Over-expression AAV
This is an AAV expressing Mouse Chmp4b.
AAV-255416
AAV-m-CHMP4B
Ready-to-use AAV expressing Mouse Chmp4b (charged multivesicular body protein 4B). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
2010012F05Rik; C76846; Snf7-2
Description (Vector)
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4B filaments can promote or stabilize negative curvature and outward budding. Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release.
Gene ID
75608
Gene Name (Vector)
charged multivesicular body protein 4B
Gene Symbol
Chmp4b
ORF Size (aa)
675
RefSeq ID
NM_029362
RefSeq Synonyms
NM_029362, BC059279, BC011429,
Species
mouse
UniGene ID
Mm.262480
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.