human PHF8 Over-expression AAV

Name 
AAV-h-PHF8
Cat No 
AAV-218824
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human PHF8.

AAV-218824
AAV-h-PHF8

Ready-to-use AAV expressing Human PHF8 (PHD finger protein 8). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
histone lysine demethylase PHF8; JHDM1F; jumonji C domain-containing histone demethylase 1F; KDM7B; MRXSSD; PHD finger protein 8; ZNF422
Description (eCommerce)
PHF8 (PHD finger protein 8) is a member of the jumonji family of proteins and contains a jumonji C (JmjC) domain. The JmjC proteins are predicted to be metalloenzymes that play a role a chromatin remodeling and histone demethylation. PHF8 also bears a PHD (plant homeodomain)- type zinc-finger, a domain also found to be involved in chromatin remodeling and transcriptional regulation. Truncating mutations in PHF8 are associated with X-linked mental retardation and cleft lip/cleft palate.
Description (Vector)
PHF8 is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. Defects in this gene are a cause of mental retardation syndromic X-linked Siderius type (MRXSSD). Four transcript variants encoding different isoforms have been found for this gene.
Gene ID
23133
Gene Name (eCommerce)
PHD finger protein 8
Gene Name (Vector)
PHD finger protein 8
Gene Symbol
PHF8
HGNC ID
HGNC:20672
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3075
ORF Size (bp)
3075 bp
Protein Name (eCommerce)
Histone lysine demethylase PHF8
RefSeq ID
NM_015107
RefSeq Synonyms
NM_015107, NM_001184898, NM_001184897, NM_001184896, BC053861,
Research Areas
Brain Development,Cancer,Cell Cycle,Chromatin Remodeling,Histone Modification,Neurobiology,Neurodevelopment,Transcription Factor/Regulator,Zinc-finger
Research Areas (Faceted)
cancer,cell_biology,cell_cycle,developmental_biology,genetics,transcription_translation,neurobiology
Species
human
Target Sentence
PHF8 (PHD finger protein 8) is a member of the jumonji family of proteins and contains a jumonji C (JmjC) domain. The JmjC proteins are predicted to be metalloenzymes that play a role a chromatin remodeling and histone demethylation. PHF8 also bears a PHD (plant homeodomain)- type zinc-finger, a domain also found to be involved in chromatin remodeling and transcriptional regulation. Truncating mutations in PHF8 are associated with X-linked mental retardation and cleft lip/cleft palate.
UniGene ID
Hs.133352
UniProt ID (eCommerce)
Q9UPP1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.