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human PHF8 shRNA silencing AAV

Name 
AAV-h-PHF8-shRNA
Cat No 
shAAV-218824
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PHF8.

shAAV-218824
AAV-h-PHF8-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PHF8 (PHD finger protein 8). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
histone lysine demethylase PHF8; JHDM1F; jumonji C domain-containing histone demethylase 1F; KDM7B; MRXSSD; PHD finger protein 8; ZNF422
Description (eCommerce)
PHF8 (PHD finger protein 8) is a member of the jumonji family of proteins and contains a jumonji C (JmjC) domain. The JmjC proteins are predicted to be metalloenzymes that play a role a chromatin remodeling and histone demethylation. PHF8 also bears a PHD (plant homeodomain)- type zinc-finger, a domain also found to be involved in chromatin remodeling and transcriptional regulation. Truncating mutations in PHF8 are associated with X-linked mental retardation and cleft lip/cleft palate.
Description (Vector)
PHF8 is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. Defects in this gene are a cause of mental retardation syndromic X-linked Siderius type (MRXSSD). Four transcript variants encoding different isoforms have been found for this gene.
Gene ID
23133
Gene Name (eCommerce)
PHD finger protein 8
Gene Name (Vector)
PHD finger protein 8
Gene Symbol
PHF8
HGNC ID
HGNC:20672
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3075
ORF Size (bp)
3075 bp
Protein Name (eCommerce)
Histone lysine demethylase PHF8
RefSeq ID
NM_015107
RefSeq Synonyms
NM_015107, NM_001184898, NM_001184897, NM_001184896, BC053861,
Research Areas
Brain Development,Cancer,Cell Cycle,Chromatin Remodeling,Histone Modification,Neurobiology,Neurodevelopment,Transcription Factor/Regulator,Zinc-finger
Research Areas (Faceted)
cancer,cell_biology,cell_cycle,developmental_biology,genetics,transcription_translation,neurobiology
Species
human
Target Sentence
PHF8 (PHD finger protein 8) is a member of the jumonji family of proteins and contains a jumonji C (JmjC) domain. The JmjC proteins are predicted to be metalloenzymes that play a role a chromatin remodeling and histone demethylation. PHF8 also bears a PHD (plant homeodomain)- type zinc-finger, a domain also found to be involved in chromatin remodeling and transcriptional regulation. Truncating mutations in PHF8 are associated with X-linked mental retardation and cleft lip/cleft palate.
UniGene ID
Hs.133352
UniProt ID (eCommerce)
Q9UPP1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.