human PFKL Over-expression AAV

Name 
AAV-h-PFKL
Cat No 
AAV-218683
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human PFKL.

AAV-218683
AAV-h-PFKL

Ready-to-use AAV expressing Human PFKL (phosphofructokinase, liver type). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
6-phosphofructokinase type B; 6-phosphofructokinase, liver type; ATP-dependent 6-phosphofructokinase, liver type; ATP-PFK; liver-type 1-phosphofructokinase; PFK-B; PFK-L; phosphofructo-1-kinase isozyme B; phosphohexokinase
Description (eCommerce)
Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. PFKL represents the L subunit [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P17858].
Description (Vector)
Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD).
Gene ID
5211
Gene Name (eCommerce)
phosphofructokinase, liver type
Gene Name (Vector)
phosphofructokinase, liver type
Gene Symbol
PFKL
HGNC ID
HGNC:8876
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2484
ORF Size (bp)
2484 bp
Protein Name (eCommerce)
ATP-dependent 6-phosphofructokinase, liver type
RefSeq ID
NM_002626
RefSeq Synonyms
NM_002626, NM_001002021, BC009919, BC008964, BC007536, BC006422,
Research Areas
HIF-1 Signaling,Insulin Signaling,Kinase/Phosphatase,Metabolism/Metabolic Process,Signal Transduction
Research Areas (Faceted)
signal_transduction,metabolism,cell_biology
Species
human
Target Sentence
Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. PFKL represents the L subunit [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P17858].
UniGene ID
Hs.255093
UniProt ID (eCommerce)
P17858

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.