human PDHB Over-expression AAV

Name 
AAV-h-PDHB
Cat No 
AAV-218518
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human PDHB.

AAV-218518
AAV-h-PDHB

Ready-to-use AAV expressing Human PDHB (pyruvate dehydrogenase (lipoamide) beta). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
PDHBD; PDHE1-B; PHE1B
Description (Vector)
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and carbon dioxide, and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 beta subunit. Mutations in this gene are associated with pyruvate dehydrogenase E1-beta deficiency. Alternatively spliced transcript variants have been found for this gene.
Gene ID
5162
Gene Name (Vector)
pyruvate dehydrogenase (lipoamide) beta
Gene Symbol
PDHB
HGNC ID
HGNC:8808
ORF Size (aa)
1080
RefSeq ID
NM_000925
RefSeq Synonyms
NM_001315536, NM_001173468, NM_000925, BC001924, BC000439,
Species
human
UniGene ID
Hs.161357

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.