AAV with CAG2 promoter driven hChR2(E123T/H134R)-TdTomato
This AAV expresses hChR2(E123T/H134R)-TdTomato driven by an ubiquitous CAG2 promoter.
The CAG2 promoter is derived from the commonly used 1.8kb CAG/CBA (also called CAGGS) promoter. To increase the cloning capacity of size-limiting vectors using the CAG promoter, such as AAV or lentiviral vectors, CAG2 was developed by deleting a portion of about 0.6Kb of the chicken beta-actin intron from the original CAG promoter.
hChR2(E123T/H134R), also known as ChETA, is a channelrhodopsin-2 variant with faster deactivation kinetics.
VB2987
AAV-CAG2-hChR2(E123T/H134R)-TdTomato
Ready-to-use AAV expressing hChR2(E123T/H134R)-TdTomato driven by an ubiquitous CAG2 promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.