AAV with ApoE/hAAT1 promoter driven Mito-roGFP1

Name 
AAV-ApoE/hAAT1-Mito-roGFP1-WPRE
Cat No 
VB2006
Availability
3 weeks
Categories
AAV, Control/Reporter, Over-Expression

This AAV expresses Mito-roGFP1 driven by a liver ApoE/hAAT1 promoter.

The ApoE/AAT1 chimeric promoter consists of the core promoter from human alpha-1-antitrypsin gene (also known as hAAT1, SERPINA1, A1A, A1AT, AAT, or alpha1AT) and the enhancer/hepatic locus control region (HCR) from the ApoE gene. The ApoE-hAAT1 chimeric promoter is about 0.7Kb and is used for driving gene expression in hepatocytes

The mitochondrial targeting sequence of human pyruvate dehydrogenase alpha 1 (PDHA1) is fused in-frame with the redox-sensitive green fluorescent protein (roGFP1). The Mito-roGFP1 is a biosensor for investigating the mitochondrial reduction-oxidation potential.

VB2006
AAV-ApoE/hAAT1-Mito-roGFP1-WPRE

Ready-to-use AAV expressing Mito-roGFP1 driven by a liver ApoE/hAAT1 promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

Request a Quote

Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
ApoE/hAAT1 (liver)
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume (µL)
200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.