AAV with mIP1 promoter driven CreERT2

Name 
AAV-mIP1-CreERT2
Cat No 
VB1774
Availability
2-3 weeks
Categories
AAV, Cre Recombinases, Over-Expression

This AAV expresses CreERT2 driven by an islet mIP1 promoter.

The mouse insulin-I promoter, (m)INS1, mIP-I or mIP1, is a promoter fragment of about 1.2 kb in length from the murine preproinsulin-I gene. It drives transgene expression in beta- cells of the pancreas, in the research field of diabetes and obesity.

A mutated form of estrogen ligand-binding domain (ERT2) that binds to synthetic antagonists (such as tamoxifen or its derivative 4-hydroxy-tamoxifen) but not to circulating estrogen, was fused to the Cre recombinase (Cre) to create CreERT2 (also known as “inducible Cre”). When tamoxifen binds to CreERT2, it induces a conformational change of CreERT2, leading to its nuclear translocation, followed by Cre/Lox-mediated recombination.

VB1774
AAV-mIP1-CreERT2

Ready-to-use AAV expressing CreERT2 driven by an islet mIP1 promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
mIP1 (islet)
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume
100 µl, 250 µl, 500 µl, and 1ml

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.