AAV with hRPE(1.5) promoter driven Cre Inducible EYFP

Name 
AAV-hRPE(1.5)-DIO-EYFP
Cat No 
VB1258
Availability
3 weeks
Categories
Cre Inducible Reporter, AAV, Over-Expression

This AAV expresses DIO-EYFP driven by a retina hRPE(1.5) promoter.

The RPE65 gene encodes a protein of the retinal pigment epithelium (RPE) with a key role in the cycling of retinoids. This 1.5Kb promoter gives RPE-specific expression, but the expression level is not as high as that of the shorter, 0.8Kb, promoter. This is probably due to a negative regulatory element(s) present in the 1.5Kb promoter.

In the DIO scenario, the transgene of interest is inserted in reverse orientation relative to the 5′ promoter and is flanked by oppositely oriented loxP and lox2272 sites. In the absence of Cre expression, the transgene will not be produced. In the presence of Cre expression, the transgene will be “FLip-EXchanged” or FLEXed, leading to expression of the transgene. This is due to a permanent Cre-mediated recombination/inversion of the flanked transgene. This arrangement is called DIO (double-floxed inverse ORF), Cre-ON, Flex-rev (reverse), Flex-ON/FlexON, or DIO-AAV/AAV-DIO (double-floxed inverse ORF in AAV).

VB1258
AAV-hRPE(1.5)-DIO-EYFP

Ready-to-use AAV expressing DIO-EYFP driven by a retina hRPE(1.5) promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

Request a Quote

Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
hRPE(1.5) (retina)
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume (µL)
200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.