rat SMAD2 shRNA silencing Adenovirus

Name 
Ad-r-SMAD2-shRNA
Cat No 
shADV-295091
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Rat Smad2.

shADV-295091
Ad-r-SMAD2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Rat Smad2 (SMAD family member 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
81
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
Madh2
Description (Vector)
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Gene ID
29357
Gene Name (Vector)
SMAD family member 2
Gene Symbol
Smad2
ORF Size (aa)
1404
ORF Size (bp)
1404 bp
RefSeq ID
NM_001277450
RefSeq Synonyms
NM_019191, NM_001277450, BC127497,
Species
rat

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.