rat TTRAP shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Rat Tdp2.
shADV-294214
Ad-r-TTRAP-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Rat Tdp2 (tyrosyl-DNA phosphodiesterase 2). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
Ttrap
Description (Vector)
DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation.
Gene ID
498749
Gene Name (Vector)
tyrosyl-DNA phosphodiesterase 2
Gene Symbol
Tdp2
ORF Size (aa)
1101
ORF Size (bp)
1101 bp
RefSeq ID
NM_001034947
RefSeq Synonyms
NM_001034947, BC101920,
Species
rat
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.