rat NFKBIB shRNA silencing Adenovirus

Name 
Ad-r-NFKBIB-shRNA
Cat No 
shADV-293805
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Rat Nfkbib.

shADV-293805
Ad-r-NFKBIB-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Rat Nfkbib (NFKB inhibitor beta). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
75
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Description (Vector)
Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
Gene ID
81525
Gene Name (Vector)
NFKB inhibitor beta
Gene Symbol
Nfkbib
ORF Size (aa)
1080
ORF Size (bp)
1080 bp
RefSeq ID
NM_030867
RefSeq Synonyms
NM_030867, BC085729,
Species
rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.