mouse UPF1 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Mouse Upf1.
shADV-275657
Ad-m-UPF1-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Upf1 (UPF1 regulator of nonsense transcripts homolog (yeast)). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
B430202H16Rik; NORF1; PNORF-1; Rent1; Upflp
Description (Vector)
RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD. Essential for embryonic viability.
Gene ID
19704
Gene Name (Vector)
UPF1 regulator of nonsense transcripts homolog (yeast)
Gene Symbol
Upf1
ORF Size (aa)
3375
ORF Size (bp)
3375 bp
RefSeq ID
NM_030680
RefSeq Synonyms
NM_030680, NM_001122829, BC056442, BC052149,
Species
mouse
UniGene ID
Mm.390048
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.