human UPF1 shRNA silencing Adenovirus

Name 
Ad-h-UPF1-shRNA
Cat No 
shADV-227223
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human UPF1.

shADV-227223
Ad-h-UPF1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human UPF1 (UPF1, RNA helicase and ATPase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
87
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ATP-dependent helicase RENT1; delta helicase; HUPF1; nonsense mRNA reducing factor 1; NORF1; pNORF1; regulator of nonsense transcripts 1; RENT1; smg-2; smg-2 homolog, nonsense mediated mRNA decay factor; UPF1 regulator of nonsense transcripts homolog; up-frameshift mutation 1 homolog; up-frameshift suppressor 1 homolog; yeast Upf1p homolog
Description (eCommerce)
RENT1, also known as UPF1, is a key regulator of the nonsense-mediated mRNA decay (NMD) pathway. The NMD pathway eliminates mRNAs that prematurely terminate during translation and may serve to protect the cells from dominant-negative effects of truncated proteins, or as a means to regulate protein expression. RENT1 is an ATP-dependent 5’- 3’ RNA helicase and ATPase that localizes to the cytoplasm and is part of a post-splicing multiprotein complex as well as an mRNA decay complex.
Description (Vector)
UPF1 encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene.
Gene ID
5976
Gene Name (eCommerce)
UPF1, RNA helicase and ATPase
Gene Name (Vector)
UPF1, RNA helicase and ATPase
Gene Symbol
UPF1
HGNC ID
HGNC:9962
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3357
ORF Size (bp)
3357 bp
Protein Name (eCommerce)
Regulator of nonsense transcripts 1
RefSeq ID
NM_002911
RefSeq Synonyms
NM_002911, NM_001297549, BC039817,
Research Areas
Cell Cycle,Chromatin Remodeling,DNA Damage/Repair,DNA Replication,Gene Expression,Helicase Activity,Nuclear Import/Export,RNA Binding,RNA Processing,RNA Transport,Spliceosome,Translational Control,Transport,Zinc-finger
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,transcription_translation,cell_biology
Species
human
Target Sentence
RENT1, also known as UPF1, is a key regulator of the nonsense-mediated mRNA decay (NMD) pathway. The NMD pathway eliminates mRNAs that prematurely terminate during translation and may serve to protect the cells from dominant-negative effects of truncated proteins, or as a means to regulate protein expression. RENT1 is an ATP-dependent 5’- 3’ RNA helicase and ATPase that localizes to the cytoplasm and is part of a post-splicing multiprotein complex as well as an mRNA decay complex.
UniGene ID
Hs.515266
UniProt ID (eCommerce)
Q92900

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.