mouse TRMU shRNA silencing Adenovirus

Name 
Ad-m-TRMU-shRNA
Cat No 
shADV-275059
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Trmu.

shADV-275059
Ad-m-TRMU-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Trmu (tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
1110005N20Rik; 1600025P05Rik; AI314320; Trmt1
Description (Vector)
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.
Gene ID
72026
Gene Name (Vector)
tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase
Gene Symbol
Trmu
ORF Size (aa)
1254
ORF Size (bp)
1254 bp
RefSeq ID
NM_028063
RefSeq Synonyms
NM_028063, BC061026,
Species
mouse
UniGene ID
Mm.158211

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.