human NANS Over-expression AAV

Name 
AAV-h-NANS
Cat No 
AAV-216303
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human NANS.

AAV-216303
AAV-h-NANS

Ready-to-use AAV expressing Human NANS (N-acetylneuraminate synthase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
HEL-S-100; SAS; SEMDCG; SEMDG
Description (Vector)
NANS encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. NANS is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant.
Gene ID
54187
Gene Name (Vector)
N-acetylneuraminate synthase
Gene Symbol
NANS
HGNC ID
HGNC:19237
ORF Size (aa)
1080
ORF Size (bp)
1080 bp
RefSeq ID
NM_018946
RefSeq Synonyms
NM_018946, BC019315, BC000008,
Species
human
UniGene ID
Hs.665191

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.