human USP15 shRNA silencing Adenovirus

Name 
Ad-h-USP15-shRNA
Cat No 
shADV-227287
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human USP15.

shADV-227287
Ad-h-USP15-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human USP15 (ubiquitin specific peptidase 15). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
deubiquitinating enzyme 15; ubiquitin carboxyl-terminal hydrolase 15; ubiquitin thioesterase 15; ubiquitin thiolesterase 15; ubiquitin-specific-processing protease 15; UNPH-2; UNPH4
Description (eCommerce)
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 15 (USP15) is a member of this family.
Description (Vector)
USP15 encodes a member of the ubiquitin specific protease (USP) family of deubiquitinating enzymes. USP enzymes play critical roles in ubiquitin-dependent processes through polyubiquitin chain disassembly and hydrolysis of ubiquitin-substrate bonds. The encoded protein associates with the COP9 signalosome, and also plays a role in transforming growth factor beta signalling through deubiquitination of receptor-activated SMAD transcription factors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 2.
Gene ID
9958
Gene Name (eCommerce)
ubiquitin specific peptidase 15
Gene Name (Vector)
ubiquitin specific peptidase 15
Gene Symbol
USP15
HGNC ID
HGNC:12613
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2859
ORF Size (bp)
2859 bp
Protein Name (eCommerce)
Ubiquitin carboxyl-terminal hydrolase 15
RefSeq ID
NM_006313
RefSeq Synonyms
NM_006313, NM_001252079, NM_001252078, BC125123,
Research Areas
Breast Cancer,Cancer,Signal Transduction,TGF-beta Signaling,Ubiquitination
Research Areas (Faceted)
cancer,cell_biology,immunology,signal_transduction
Species
human
Target Sentence
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 15 (USP15) is a member of this family.
UniGene ID
Hs.434951
UniProt ID (eCommerce)
Q9Y4E8

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.