human NAGPA Over-expression AAV

Name 
AAV-h-NAGPA
Cat No 
AAV-216292
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human NAGPA.

AAV-216292
AAV-h-NAGPA

Ready-to-use AAV expressing Human NAGPA (N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
APAA; UCE
Description (Vector)
Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. This reaction most likely occurs in the trans-Golgi network. This enzyme functions as a homotetramer of two disulfide-linked homodimers. In addition to having an N-terminal signal peptide, the protein's C-terminus contains multiple signals for trafficking it between lysosomes, the plasma membrane, and trans-Golgi network.
Gene ID
51172
Gene Name (Vector)
N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase
Gene Symbol
NAGPA
HGNC ID
HGNC:17378
ORF Size (aa)
930
ORF Size (bp)
930 bp
RefSeq ID
NM_016256
RefSeq Synonyms
NM_016256, BC012194,
Species
human
UniGene ID
Hs.21334

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.