mouse PIWIL4 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Mouse Piwil4.
shADV-268721
Ad-m-PIWIL4-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Piwil4 (piwi-like RNA-mediated gene silencing 4). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
9230101H05Rik; Miwi2
Description (Vector)
Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci.
Gene ID
330890
Gene Name (Vector)
piwi-like RNA-mediated gene silencing 4
Gene Symbol
Piwil4
ORF Size (aa)
2637
ORF Size (bp)
2637 bp
RefSeq ID
NM_177905
RefSeq Synonyms
NM_177905, BC153016, BC148393,
Species
mouse
UniGene ID
Mm.334969
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.