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mouse MLF1 shRNA silencing Adenovirus

Name 
Ad-m-MLF1-shRNA
Cat No 
shADV-264686
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Mouse Mlf1.

shADV-264686
Ad-m-MLF1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Mlf1 (myeloid leukemia factor 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
HLS7
Description (Vector)
Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
Gene ID
17349
Gene Name (Vector)
myeloid leukemia factor 1
Gene Symbol
Mlf1
ORF Size (aa)
849
ORF Size (bp)
849 bp
RefSeq ID
NM_010801
RefSeq Synonyms
NM_010801, NM_001039543, BC027526,
Species
mouse
UniGene ID
Mm.10414

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.