mouse HUS1 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Mouse Hus1.
shADV-261797
Ad-m-HUS1-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Hus1 (HUS1 checkpoint clamp component). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Description (Vector)
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of C12orf32/RHINO to sites of double-stranded breaks (DSB) occurring during the S phase.
Gene ID
15574
Gene Name (Vector)
HUS1 checkpoint clamp component
Gene Symbol
Hus1
ORF Size (aa)
903
ORF Size (bp)
903 bp
RefSeq ID
NM_008316
RefSeq Synonyms
NM_008316, NM_001303610, NM_001303532, BC061249,
Species
mouse
UniGene ID
Mm.42201
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.