human HUS1 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human HUS1.
shAAV-211721
AAV-h-HUS1-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human HUS1 (HUS1 checkpoint clamp component). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
hHUS1
Description (Vector)
HUS1 is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants.
Gene ID
3364
Gene Name (Vector)
HUS1 checkpoint clamp component
Gene Symbol
HUS1
HGNC ID
HGNC:5309
ORF Size (aa)
843
ORF Size (bp)
843 bp
RefSeq ID
NM_004507
RefSeq Synonyms
NM_004507, BC007013,
Species
human
UniGene ID
Hs.152983
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.