mouse SAP18 shRNA silencing Adenovirus

Name 
Ad-m-SAP18-shRNA
Cat No 
shADV-258428
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Sap18.

shADV-258428
Ad-m-SAP18-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Sap18 (Sin3-associated polypeptide 18). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
C530046K05Rik; D11Ertd539e; EMegR4; Sap18a; Sinbp1
Description (Vector)
Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism.
Gene ID
20220
Gene Name (Vector)
Sin3-associated polypeptide 18
Gene Symbol
Sap18
ORF Size (aa)
462
ORF Size (bp)
462 bp
RefSeq ID
NM_009119
RefSeq Synonyms
NM_009119, BC099480, BC006625,
Species
mouse
UniGene ID
Mm.344671

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.