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mouse DOT1L shRNA silencing Adenovirus

Name 
Ad-m-DOT1L-shRNA
Cat No 
shADV-257398
Availability
Immediate

This is an Adenovirus expressing shRNA for silencing of Mouse Dot1l.

shADV-257398
Ad-m-DOT1L-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Dot1l (DOT1-like, histone H3 methyltransferase (S. cerevisiae)). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
A630076O07; AW907654; Dot1; KMT4; mDot1
Description (Vector)
Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.
Gene ID
208266
Gene Name (Vector)
DOT1-like, histone H3 methyltransferase (S. cerevisiae)
Gene Symbol
Dot1l
ORF Size (aa)
4623
ORF Size (bp)
4623 bp
RefSeq ID
NM_199322
RefSeq Synonyms
NM_199322, BC167196,
Species
mouse
UniGene ID
Mm.393160

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.