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human DOT1L shRNA silencing AAV

Name 
AAV-h-DOT1L-shRNA
Cat No 
shAAV-207261
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human DOT1L.

shAAV-207261
AAV-h-DOT1L-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human DOT1L (DOT1 like histone lysine methyltransferase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
60
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
DOT1; DOT1 like histone H3K79 methyltransferase; DOT1-like histone methyltransferase; DOT1-like protein; DOT1-like, histone H3 methyltransferase; H3-K79-HMTase; histone H3-K79 methyltransferase; histone methyltransferase DOT1L; histone-lysine N-methyltransferase, H3 lysine-79 specific; KMT4; lysine N-methyltransferase 4
Description (eCommerce)
DOT1L is a histone methyltransferase that methylates nucleosomal histone H3 on lysine 79. In contrast to other lysine histone methyltransferase, DOT1L does not contain a SET domain, a domain shown to be necessary for methyltransferase activity.
Description (Vector)
DOT1L is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes.
Gene ID
84444
Gene Name (eCommerce)
DOT1 like histone lysine methyltransferase
Gene Name (Vector)
DOT1 like histone lysine methyltransferase
Gene Symbol
DOT1L
HGNC ID
HGNC:24948
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
4611
ORF Size (bp)
4611 bp
Protein Name (eCommerce)
Histone-lysine N-methyltransferase, H3 lysine-79 specific
RefSeq ID
NM_032482
RefSeq Synonyms
NM_032482, BC156103,
Research Areas
Cancer,Chromatin Remodeling,Histone Modification,JAK-STAT Signaling,Methyltransferase,Neurobiology,Signal Transduction,Transcription Factor/Regulator,Transcriptional Misregulation in Cancer
Research Areas (Faceted)
cancer,signal_transduction,cell_biology,transcription_translation,genetics,signal_transduction,cell_biology,neurobiology,genetics,transcription_translation,cancer
Species
human
Target Sentence
DOT1L is a histone methyltransferase that methylates nucleosomal histone H3 on lysine 79. In contrast to other lysine histone methyltransferase, DOT1L does not contain a SET domain, a domain shown to be necessary for methyltransferase activity.
UniGene ID
Hs.713641
UniProt ID (eCommerce)
Q8TEK3

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.