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mouse DGKH shRNA silencing Adenovirus

Name 
Ad-m-DGKH-shRNA
Cat No 
shADV-257121
Availability
7-8 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Dgkh.

shADV-257121
Ad-m-DGKH-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Dgkh (diacylglycerol kinase, eta). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
5930402B05Rik; D130015C16; DGK
Description (Vector)
Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization.
Gene ID
380921
Gene Name (Vector)
diacylglycerol kinase, eta
Gene Symbol
Dgkh
ORF Size (aa)
3237
ORF Size (bp)
3237 bp
RefSeq ID
NM_001081336
RefSeq Synonyms
NM_001281794, NM_001253766, NM_001081336, BC127173,
Species
mouse
UniGene ID
Mm.411737

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.