mouse CHM shRNA silencing Adenovirus

Name 
Ad-m-CHM-shRNA
Cat No 
shADV-255407
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Mouse Chm.

shADV-255407
Ad-m-CHM-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Chm (choroidermia (RAB escort protein 1)). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
Rep-1
Description (Vector)
Binds unprenylated Rab proteins, presents it to the catalytic Rab GGTase dimer, and remains bound to it after the geranylgeranyl transfer reaction. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Also a pre-formed complex consisting of CHM and the Rab GGTase dimer (RGGT or component B) can bind to and prenylate Rab proteins; this alternative pathway is proposed to be the predominant pathway for Rab protein geranylgeranylation.
Gene ID
12662
Gene Name (Vector)
choroidermia (RAB escort protein 1)
Gene Symbol
Chm
ORF Size (aa)
1998
ORF Size (bp)
1998 bp
RefSeq ID
NM_018818
RefSeq Synonyms
NM_018818, BC047461,
Species
mouse
UniGene ID
Mm.257316

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.