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mouse CENPT shRNA silencing Adenovirus

Name 
Ad-m-CENPT-shRNA
Cat No 
shADV-255275
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Mouse Cenpt.

shADV-255275
Ad-m-CENPT-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Cenpt (centromere protein T). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
G630055P03Rik
Description (Vector)
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPT has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis.
Gene ID
320394
Gene Name (Vector)
centromere protein T
Gene Symbol
Cenpt
ORF Size (aa)
1548
ORF Size (bp)
1548 bp
RefSeq ID
NM_177150
RefSeq Synonyms
NM_177150, BC121824,
Species
mouse
UniGene ID
Mm.334775

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.